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Detected by immunoblotting with anti-DnaJ serum (supplemental Fig. S3B). DnaJ and the rabbit immunoglobulin heavy chain were quantified, and the ratios of DnaJ to immunoglobulin heavy chain were calculated. No 32 was added to the mixture for control ( ). C and D, 32 (0.4 M) was mixed with DnaK (1.6 M) and DnaJ (0.8 M) in the presence of 1 mM ATP. Co-precipitated DnaJ (C) (supplemental Fig. S3C) an